Validation of an immunoperoxidase monolayer assay for total anti–Vaccinia virus antibody titration

Standardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody.

An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...

Titration of Rinderpest Virus and Its Neutralizing Antibody

titration of rinderpest virus and its neutralizing antibody

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standardization of an enzyme-linked immunosorbent assay for detection of infectious bronchitis virus antibody.

an indirect enzyme–linked immunosorbent assay (elisa) was developed for screening of antibody to avian infectious bronchitis virus (ibv). antigen was prepared from whole-purified ibv massachusetts serotype (br 801 strain). optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed elisa was determined 0.1μg/ml, 1:3000 and 1:100, respe...

An infectivity assay for bovine leukemia virus using the immunoperoxidase technique.

This report describes the development of a 7-day infectivity assay for bovine leukemia virus (BLV) which is based on the induction of the major internal virion antigen (a protein with a molecular weight of 25,000) in susceptible indicator monolayer cell cultures. In this assay, the antigen is detected in the indicator cells by the immunoperoxidase antibody technique using a specific rabbit anti...